A postdoc position is available in the van Wijk lab concerning protein stability in plant chloroplasts. The project concerns the generation of a quantitative reporter system for in vivo testing of an N-end rule for protein stability in plant chloroplasts. This involves cloning, Arabidopsis transformation and transgenics, protein biochemistry and a quantitative confocal microscopy using state-of the art instrumentation. Applications welcome immediately until the position is filled.
Background: Protein amino (N) termini are prone to modifications and are major determinants of protein stability in bacteria, eukaryotes, and perhaps also in plant chloroplasts. The role of the N-terminus in protein stability is conceptualized in the N-end rule, which states that certain amino acids, when exposed at the N-terminus of a protein, act as triggers (degrons) for degradation. Based on several types of indirect experimental evidence, the presence of the Clp protease system including a ClpS homolog, as well as evolutionary arguments, we postulate that an N-end rule in plastids must exist. This project will build a quantitative reporter system to directly test possible N-degrons in chloroplasts and the involvement of chloroplast ClpS1 and the Clp protease and chaperone system in Arabidopsis.
Applicants must have a PhD, publications in competitive journals, and demonstrated experience in molecular cloning, Arabidopsis transformation and transgenics. Experience with (quantitative) confocal microscopy is highly beneficial.
Internal Number: 2018-v1
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